The limit of resolution of an oil immersion objective microscope of numerical aperture 0.8 for light of wavelength 0.6 μ m is

Question

The limit of resolution of an oil immersion objective microscope of numerical aperture 0.8 for light of wavelength 0.6 μ m is (A) (1.5/8) μ m (B) (3/8) μ m (C) (5/8) μ m (D) (7/8) μ m

Tardigrade Admin · Accepted Answer

Numerical aperture, N A=0.8 Wavelength, λ=0.6 μ m ∴ Limit of resolution =(λ/2 N A)=(0.6/2 × 0.8)=(0.3/0.8)=(3/8) μ m

Q. The limit of resolution of an oil immersion objective microscope of numerical aperture $0.8$ for light of wavelength $0.6 μ m$ is

$\frac{1.5}{8} μ m$

$\frac{3}{8} μ m$

$\frac{5}{8} μ m$

$\frac{7}{8} μ m$

Numerical aperture, $N A = 0.8$
Wavelength,
$λ = 0.6 μ m$
$∴$ Limit of resolution
$= \frac{λ}{2 N A} = \frac{0.6}{2 \times 0.8} = \frac{0.3}{0.8} = \frac{3}{8} μ m$

Q. The limit of resolution of an oil immersion objective microscope of numerical aperture 0.8 for light of wavelength 0.6μm is

81.5​μm

83​μm

85​μm

87​μm