Significance of "heat shock" method in bacterial transformation is to facilitate

Question

Significance of "heat shock" method in bacterial transformation is to facilitate (A) Binding of DNA to the cell wall (B) Uptake of DNA through membrane transport proteins (C) Uptake of DNA through transient pores in the bacterial cell wall (D) Expression of antibiotic resistance gene

Tardigrade Admin · Accepted Answer

Heat shock method in presence of a bivalent cation like

C a^{+ 2}

creates transient pores in the cell wall allowing the uptake of foreign DNA present in the medium.
So, the correct answer is 'Uptake of DNA through transient pores in the bacterial cell membrane'

Q. Significance of "heat shock" method in bacterial transformation is to facilitate

Binding of DNA to the cell wall

Uptake of DNA through membrane transport proteins

Uptake of DNA through transient pores in the bacterial cell wall

Expression of antibiotic resistance gene

Heat shock method in presence of a bivalent cation like Ca+2 creates transient pores in the cell wall allowing the uptake of foreign DNA present in the medium. So, the correct answer is 'Uptake of DNA through transient pores in the bacterial cell membrane'

Heat shock method in presence of a bivalent cation like $C a^{+ 2}$ creates transient pores in the cell wall allowing the uptake of foreign DNA present in the medium.
So, the correct answer is 'Uptake of DNA through transient pores in the bacterial cell membrane'