Question

Select the incorrect statement.

• A. Insertional inactivation technique helps in identification of the recombinant transformants with non-transformants by forming colourless colonies.
• B. Insertional inactivation of $\beta -$ galactosidase leads to the formation of colourless colonies.
• C. Insertional inactivation of $\beta -$ galactosidase leads to the formation of blue color colonies.
• D. In insertional inactivation, the $\text{rDNA}$ is inserted within the coding sequence of an enzyme $\beta -$ galactosidase.

Answer 1

Answer: (C)

Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure. Therefore, alternative selectable markers have been developed, which differentiate recombinants from non-recombinants on the basis of their ability to produce colour. A recombinant undefined is inserted within the coding sequence of enzyme undefinedgalactosidase. This results in insertational inactivation, and the colonies do not produces any colour.