Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites, ori, antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution, the separated bands of DNA are cut out from agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product.

Question

Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites, ori, antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution, the separated bands of DNA are cut out from agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product. (A) All sentences are correct (B) (I) and (III) are correct (C) Except (II), all are correct (D) Only (III) is correct

Tardigrade Admin · Accepted Answer

Vector pBR 322 have 8 restriction enzyme recognition site, ori, antibiotic resistant genes (Amphicilin and tertacyclin) which function as selectable marker and rop. Competent bacterial cell can take up foregin DNA. The extraction of DNA from gel block after gel electrophoresis is called as elution.