Q. Read the following four statements (A-D) about certain mistakes in two of them :
(A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched.
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules
(C) Downstream processing is one of the steps of R-DNA technology
(D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host
Which are the two statements having mistakes ?

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Solution:

In 1997, the first transgenic cow, Rosie, produced human protein enriched milk. The milk contained the human alpha-lactalbumin and was nutritionally a more balanced product for human babies than natural cow-milk. Isolation of DNA from other macromolecule is achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).