Primers used in PCR must be:

Question

Primers used in PCR must be: (A) 3'-end specific (B) 5'-end specific (C) It can be 3'-end specific or 5'-end specific. (D) Primers are not needed in PCR.

Tardigrade Admin · Accepted Answer

Since the replication of DNA occurs in a 5' to 3' direction, the primers used should be complementary to 3' end of template DNA. The primers used in PCR are the small primers of DNA.

Q. Primers used in PCR must be:

3'-end specific

5'-end specific

It can be 3'-end specific or 5'-end specific.

Primers are not needed in PCR.

Since the replication of DNA occurs in a 5' to 3' direction, the primers used should be complementary to 3' end of template DNA. The primers used in PCR are the small primers of DNA.