Biodiesel is formed from vegetable oil, animal oils etc. This is due to esterification of triglyceride. This is the process by which esters are formed due to reaction between alcohols and fatty acids. Sewage is a source of biomass energy. This energy is produced by anaerobic digestion and this produces Biogas. For this the raw material used is sewage sludge. Development of fruits without fertilization is known as parthenocarpy. These fruits have longer shelf life. Polyethylene glycol is used as a chemical method. This brings about gene transfer directly i.e. without using any vector.