Plasmid pBR322 has PstI restriction enzyme site within gene a m pR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain

Question

Plasmid pBR322 has PstI restriction enzyme site within gene a m pR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain (A) it will not be able to confer ampicillin resistance to the host cell. (B) the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside. (C) it will lead to lysis of host cell. (D) it will be able to produce a novel protein with dual ability.

Tardigrade Admin · Accepted Answer

Since the gene is inserted at Pst I of a m pR region of pBR322, the a m pR gene is inactivated which is called as insertional inactivation. Hence the genetically modified E.coli strain will not be able to confer ampicillin resistance.

Q. Plasmid $pBR 322$ has PstI restriction enzyme site within gene $am p^{R}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $β$ -galactoside production and the recombinant plasmid is inserted in an E.coli strain

it will not be able to confer ampicillin resistance to the host cell.

the transformed cells will have the ability to resist ampicillin as well as produce $β$ -galactoside.

it will lead to lysis of host cell.

it will be able to produce a novel protein with dual ability.

Since the gene is inserted at Pst I of $am p^{R}$ region of pBR322, the $am p^{R}$ gene is inactivated which is called as insertional inactivation. Hence the genetically modified E.coli strain will not be able to confer ampicillin resistance.

Q. Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain