PCR continues in three particular steps administered by temperature in the order of

Question

PCR continues in three particular steps administered by temperature in the order of  (A) Extension, annealing, denaturation (B) Denaturation, annealing, extension (C) Annealing, extension, denaturation (D) Denaturation, extension, annealing

Tardigrade Admin · Accepted Answer

PCR consists of three successive steps: (i) Denaturation, (ii) Primer annealing and (iii) Extension of primers. In denaturation, the temperature of 93-95 ° C is maintained for melting the two strands of a DNA molecule. In annealing, the primers attach to the target sequence of DNA. In extension, new strands of DNA are synethesised using Taq polymerase.

Q. PCR continues in three particular steps administered by temperature in the order of ______

Extension, annealing, denaturation

Denaturation, annealing, extension

Annealing, extension, denaturation

Denaturation, extension, annealing