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  4. PCR continues in three particular steps administered by temperature in the order of

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Q. PCR continues in three particular steps administered by temperature in the order of ______

NTA AbhyasNTA Abhyas 2020

Solution:

PCR consists of three successive steps: (i) Denaturation, (ii) Primer annealing and (iii) Extension of primers. In denaturation, the temperature of 93-95 $^\circ $ C is maintained for melting the two strands of a DNA molecule. In annealing, the primers attach to the target sequence of DNA. In extension, new strands of DNA are synethesised using Taq polymerase.