Question

Organelles can be separated from cell homogenate through

• A. chromatography
• B. X-rays diffraction
• C. differential centrifugation
• D. auto-radiography.

Answer 1

Answer: (C)

Organelles can be separated from cell homogenate through differential centrifugation. The basic principle involved here is sedimentation of particles in a suspension by centrifugal force. In a centrifuge, the particles sediment at different rates when an accelerating force is subjected. The rate of sedimentation depends upon the size of the particles, its shape and density.