B−DNA is an antiparallel double helix. The double strand or duplex is coiled plectonemically in right handed fashion around a common axis like a rope stair case twisted in a spiral. The coiling produces alternate major and minor grooves. One turn of 360∘ of the spiral has about 10 nucleotides on each strand of DNA. It occupies a distance of about 3.4nm(34A˚) i.e., pitch of DNA is 34A˚ so that adjacent nucleotides or their bases are separated by a space 0.34nm(3.4A˚).