Question

Km value of an enzyme is substrate concentration at

• A. $\frac{1}{4}{V}_{\max }$
• B. $2V_{\max }$
• C. $\frac{1}{2}{V}_{\max }$
• D. $V_{\max }$

Answer 1

Answer: (C)

The Michaelis constant $(K_m)$ is the concentration of substrate at which the enzyme gives an observed reaction rate that is one-half $V_{\max }$ .