Q.
Insulin (C2āH10āO5ā)nā is dissolved in a suitable solvent
and the osmotic pressure (Ļ) of solutions of various concentrations (g/cc) C is measured at 20āC. The slope
of the plot of Ļ against āCā is found to be 4.65Ć10ā3 The molecular weight of insulin is
ĻV=mBāwBāāĆRT Ļ=(VwBāā)mBāRTā Ļ=CĆmBāRTāĆ1000ā¦(i)
where C = concentration in g/cc,
Comparing eqs. (i) and (ii), ...(ii)
Slope =mRāRTāĆ1000 mBā=SlopeRTāĆ1000 =4.65Ć10ā30.0821Ć293Ć1000ā =5.17Ć106J