Q. In order to obtain virus-free plants through tissue culture the best method is

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Solution:

In meristem culture, the shoot apical meristem along with some surrounding tissue is grown in vitro. It is used for clonal propagation and recovery of virus free plants. It is also potentially useful in germ plasm exchange and long term storage of germ plasm through freeze preservation. Protoplast culture involves the culture of protoplasts (plant cell without cell wall) on suitable medium. Embryo rescue is the culture of incised immature embryo to raise new plants. Another culture involves the culture of anthers on artificial culture medium. It is helpful in obtaining haploid plants.