In Amoeba and Paramoecium, osmoregulation occurs through contractile vacoule. Osmoregulation is a phenomenon in which contractile vacuole plays an important role in maintaining the water balance of the cell. Paramoecium contains two contractile vacuoles which have fixed position. One contractile vacuole is present near the anterior end while another is present towards posterior end of the body. Each contractile vacuole is surrounded by 5-12 radial canals. Excess of water is transferred from the cytoplasm to the radial canals. The latter pour water into the contractile vacuole. The contractile vacuole expels water outside the body. Thus the contractile vacuoles and radial canals are for osmoregulation. In Amoeba the endoplasm, at its posterior end, contains a single, clear rounded and pulsating contractile vacuole, filled with a watery fluid and enclosed by a unit membrane. Surrounding this membrane is a region containing many tiny feeder vacuoles and mitochondria. It helps in the osmoregulatory and excretory activities of the animal.