Purine and pyrimidine nitrogenous base is found in DNA. Among purines, adenine pairs only with the pyrimidine thymine. Similarly, guanine pairs with the cytosine. The fact that the total amount of purine will be equal to the total amount of pyrimidine was first enunciated by Chargaff in 1950. From this law,
Adenine=Thymine=60/2=30%
Guanine=Cytosine=40/2=20%