Question

Identify the wrong statement with regard to Restriction Enzymes.

• A. Each restriction enzyme functions by inspecting the length of a DNA sequence.
• B. They cut the strand of DNA at palindromic sites.
• C. They are useful in genetic engineering.
• D. Sticky ends can be joined by using DNA ligases.

Answer 1

Answer: (B)

Restriction endonucleases make cuts at specific positions within the DNA. They function by inspecting the length of a DNA sequence. Restriction endonuclease binds to the DNA and cuts the two strands of the double helix at specific points in their sugar-phosphate backbones. They are used in genetic engineering to form recombinant molecules of DNA. DNA ligases join the DNA fragments