Question

Ideal cloning vector should have

• A. Restriction sites
• B. Selectable marker
• C. Origin of replication
• D. All of these

Answer 1

Answer: (D)

Generally, an ideal vector possesses certain characteristics:
1) Origin of Replication (ori) should be present so that the vector is capable of autonomous replication inside the host organism.
2) Presence of multiple restriction sites for insertion of DNA molecule.
3) A selectable marker should be present to screen the recombinant organism (eg. antibiotic resistance gene).
4) The vector should be smaller in size for easy incorporation into the host machinery.