Given below are some steps for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium. (i) Transform bacteria with recombinant DNA molecule. (ii) Cut the plasmid DNA using restriction enzymes. (iii) Extract plasmid DNA from bacterial cells. (iv) Bind the plasmid DNA to non-plasmid DNA fragments. (v) Use ligase to seal plasmid DNA to non-plasmid DNA. Arrange the above steps in the right sequence and select the CORRECT option.

Question

Given below are some steps for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium. (i) Transform bacteria with recombinant DNA molecule. (ii) Cut the plasmid DNA using restriction enzymes. (iii) Extract plasmid DNA from bacterial cells. (iv) Bind the plasmid DNA to non-plasmid DNA fragments. (v) Use ligase to seal plasmid DNA to non-plasmid DNA. Arrange the above steps in the right sequence and select the CORRECT option. (A) iv, v, i, ii, iii (B) iii, ii, iv, v, i (C) iii, iv, v, i, ii (D) ii, iii, v, iv, i

Tardigrade Admin · Accepted Answer

The first step in Recombinant DNA technology is isolation of the genetic material. The second step in is cutting of DNA at specific sites. The purified DNA molecules are incubated with restriction enzymes at optimal conditions for carrying out restriction enzyme digestions. The progress of a restriction enzyme digestion is checked with the help of Agarose gel electrophoresis. DNA being a negatively charged molecule moves towards the positive electrode. Similar process is repeated with the vector DNA. The 'gene of interest' obtained from the source DNA and also vector DNA obtained by restriction enzyme digestion are mixed and then ligase is added to it. This ligated DNA is inserted into recipient cell.