In C4 plants initial CO2 fixation occurs in mesophyll cells of leaf. The first CO2 acceptor is a 3-C compound Phosphoenol Pyruvate (PEP). It combines with CO2 in the presence of PEP carboxylase to form 4-C compound oxaloacetic acid. PEP+CO2+H2OPEPcarboxylase oxaloacetic acid +H3PO4 Oxaloacetic acid is reduced to malic acid, Oxaloacetic-acid +NADPHdehydrcgenas malic acid +NADP+ In C3 plants the first CO2 acceptor is ribulose 1-5 biphosphate. It combines with CO2 to produce a transient intermediate compound called 2-carboxy 3-keto 1, 5 biphosphoribotol. It immediately splits to form two molecules of 3 phosphoglyceric acid or PGA. RuBP +CO2RuBPCarboxylase 2-carboxy, 3-keto, 1, 5-biphosphoribotol 2 carboxy 3-keto 1-5 biphosphoribotol +H2O2PGA .