Question

Experiments $1,2,$ and $3$ were conducted wherein synthetic vesicles containing $F_0-F_{1}ATP$ synthase were prepared and incubated overnight in a tube. Subsequently, the vesicles were transferred to another tube which also contained $ADP$ and $Pi$ (inorganic phosphate)
(A). A proton gradient across the vesicular membrane will be present in both experiments 1 and 2 at the time of transfer.
(B). As a consequence of the proton gradient, ATP will be synthesised in both experiments 1 and 2.
(C). ATP will be synthesised in experiment 3 because $Fo-F1ATP$ synthase has the inherent property to catalyse the Synthesis of ATP from ADP and Pi.
(D). ATP will be synthesised in experiment 2 because the proton has to flow Out of the vesicles through the $F_0-F_{1}ATP$ synthase for ATP synthesis.

• A. $A$ and $B$
• B. $B$ and $C$
• C. $C$ and $D$
• D. $A$ and $D$

Answer 1

Answer: (D)

Correct answer is (d) $A$ and $D$