Protein synthesis is the process of manufacturing protein from DNA involving two steps - transcription and translation. Transcription is the process of making mRNA from the DNA and this mRNA is translated into a long chain polypeptide with help of ribosome and tRNA. The strand of DNA from which the new mRNA is synthesized is called as template strand or non-coding or anti-sense strand. It is in the 3' → 5' direction. The mRNA is synthesized on it in the 5' → 3' with the help of DNA dependent RNA polymerase enzyme which leads in the same direction.