ELISA assay

Question

ELISA assay (A) uses complement mediated cell lysis (B) uses a radiolabelled second antibody (C) involves addition of substrate which is convened into coloured end product (D) requires red blood cells

Tardigrade Admin · Accepted Answer

In ELISA (Enzyme Linked Immunosorbent Assay), the antibody is bound to an inert polymer support than exposed to the sample. The second antibody used is one that has an enzyme attached to it that converts a colourless or nonfluorescent substrate into a coloured or fluorescent product.

Q. ELISA assay

uses complement mediated cell lysis

uses a radiolabelled second antibody

involves addition of substrate which is convened into coloured end product

requires red blood cells

In ELISA (Enzyme Linked Immunosorbent Assay), the antibody is bound to an inert polymer support than exposed to the sample. The second antibody used is one that has an enzyme attached to it that converts a colourless or nonfluorescent substrate into a coloured or fluorescent product.