During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

Question

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first? (A) Annealing (B) Extension (C) Denaturation (D) Ligation

Tardigrade Admin · Accepted Answer

During PCR process, if high temperature is not maintained, the initial step of the process known as denaturation of DNA is not done.

Q. During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

Annealing

Extension

Denaturation

Ligation

During $PCR$ process, if high temperature is not maintained, the initial step of the process known as denaturation of DNA is not done.