Question

During the analysis of the DNA of an organism having 5386 nucleotides, it was discovered that the percentages of the bases were $A=29\%,G=17\%,C=32\%andT=17\%$ . Considering the Chargaff's rule it can be concluded that:

• A. It is a double-stranded linear DNA.
• B. It is a double-stranded circular DNA.
• C. It is a single-stranded DNA.
• D. Both $\left(A\right)and\left(B\right)$

Answer 1

Answer: (C)

According to Chargaff's rule in any double stranded DNA $\frac{A}{T}=1and\frac{G}{C}=1$ . That is in any double stranded DNA Amount of Adenine and Thymine should be same, as well as amount of Guanine and Cytosine should be same. Although amount $A+\frac{T}{G}+C$ is mostly not equal to 1, it is called Dissymmetry ratio.