Q. During nitration of benzene with nitrating mixture, acts as

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Solution:

Electrophilic substitution reactions involve two steps, cleavage of the or step (i.e. proton elimination) take place in the second (fast) step, which being fast does not affect the rate of reaction. The first (slow) step is the formation of carbocation in which or bond is not affected. Thus absence of isotopic effect establishes the two-steps mechanism for electrophilic aromatic substitution in which formation of carbocation (first step) is rate determining step.