In human beings, the presence of a Y- chromosome is required for the development of a male sex phenotype. X-chromosomes present in any number (XXX,XXXX) in the absence of a Y-chromosome gives rise to female. Presence of even a single Y-chromosome confers maleness. In Drosophila, Y-chromosome plays no significant role in sex determination. In Drosophila male determiners were located on autosomes. One X and two autosomes produce male while two X(X,X) and two autosomes produce female.