Choose the option that is showing the correct sequence of events occurring in each cycle of polymerase chain reaction (PCR).

Question

Choose the option that is showing the correct sequence of events occurring in each cycle of polymerase chain reaction (PCR). (A) Denaturation, extension, primer annealing. (B) Primer annealing, denaturation, extension. (C) Denaturation, primer annealing, extension. (D) Extension, primer annealing, denaturation.

Tardigrade Admin · Accepted Answer

The purpose of a PCR (Polymerase Chain Reaction) is to make a huge number of copies of a gene. There are three major steps in a PCR, which are repeated for 30 or 40 cycles. This is done on an automated cycler, which can heat and cool the tubes with the reaction mixture in a very short time. The schematic representation of PCR is as follows:
Solution

Q. Choose the option that is showing the correct sequence of events occurring in each cycle of polymerase chain reaction (PCR).

Denaturation, extension, primer annealing.

Primer annealing, denaturation, extension.

Denaturation, primer annealing, extension.

Extension, primer annealing, denaturation.