Assertion: PCR primers must not have self complementary regions. Reason: Self complementary regions result in hairpin structures adversely affecting the PCR.

Question

Assertion: PCR primers must not have self complementary regions. Reason: Self complementary regions result in hairpin structures adversely affecting the PCR. (A) If both assertion and reason are true and reason is the correct explanation of assertion (B) If both assertion and reason are true but reason is not the correct explanation of assertion (C) If assertion is true but reason is false (D) If both assertion and reason are false

Tardigrade Admin · Accepted Answer

Primers are nucleotide sequences that serve as base for formation of new DNA strand. In PCR, primers are added on the ends of DNA strand to be copied and the primers are extended towards each other so that the DNA segment lying between the two primers is copied. If self complementary bases are present in primers than hydrogen bonds are formed between them and hairpin-like structure is formed. It makes the primers unsuitable for extension of strands in PCR.

Q. Assertion : PCR primers must not have self complementary regions. Reason : Self complementary regions result in hairpin structures adversely affecting the PCR.

If both assertion and reason are true and reason is the correct explanation of assertion

If both assertion and reason are true but reason is not the correct explanation of assertion

If assertion is true but reason is false

If both assertion and reason are false

Q. Assertion : $PCR$ primers must not have self complementary regions.
Reason : Self complementary regions result in hairpin structures adversely affecting the $PCR$ .