Q. Assertion: One mole of a substance always contain the same number of entities, no matter what the substance may be
Reason: One mole is the amount of a substance that contains as many particles or entities as there are atoms in exactly of the isotope.

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Solution:

One mole of a substance always contains the same number of entities, no matter what the substance may be which is . This is also the number of atoms present in of isotope.
Both Assertion and Reason are correct and Reason is the correct explanation for Assertion