Q. Assertion: Hardy-Weinberg principle states that in the absence of disturbing influences, gene frequencies of large populations of sexually reproducing organisms do not change, provided that matings, occur at random.
Reason : The disturbing influences include mutation, gene flow, genetic drift, genetic recombination and natural selection.

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Solution:

According to Hardy-Weinberg principle gene frequencies will remain constant if there is no mutation, no gene flow, no genetic drift, no genetic recombination, no natural selection pressure and mating occurs at random.