Assertion: ELISA test is based on antigen-antibody interactions where a pathogen can be detected by the presence of antibodies (proteins, glycoproteins, etc.) on it. Reason: The pathogen antibody to be identified is immobilised on the surface of specially constructed ELISA plates and is then tested.

Question

Assertion: ELISA test is based on antigen-antibody interactions where a pathogen can be detected by the presence of antibodies (proteins, glycoproteins, etc.) on it. Reason: The pathogen antibody to be identified is immobilised on the surface of specially constructed ELISA plates and is then tested. (A) If both assertion and reason are true and reason is the correct explanation of assertion (B) If both assertion and reason are true but reason is not the correct explanation of assertion (C) If assertion is true but reason is false (D) If both assertion and reason are false

Tardigrade Admin · Accepted Answer

ELISA (enzyme linked immuno-sorbant assay) is a technique based on the principle of antigen-antibody interactions. Pathogens have specific antigens on their surface like protein, glycoproteins, etc. Thus, their infection can be detected by using the antibodies specific to bind with those antigens. In process antigens are immobilised on ELISA plates. Then antibody specific to the antigen is added and allowed to react with the adsorbed antigen. A complex procedure is followed.