Arrange the steps of PCR in decreasing order of temperature.

Question

Arrange the steps of PCR in decreasing order of temperature. (A) Denaturation < Polymerisation < Annealing (B) Polymerisation < Denaturation < Annealing (C) Polymerisation < Annealing < Denaturation (D) Annealing < Polymerisation < Denaturation

Tardigrade Admin · Accepted Answer

PCR is Polymerase chain reaction. This technique is used to form numerous copies of a specific segment of DNA. Thus large quantities of DNA are obtained that are required for various experiments in medicine, molecular biology, forensic biology etc. The steps are:

1.

Denaturation of the template into single strands. The temperature is 94 degree celsius.

2.

Annealing of primers to each original strand for new strand synthesis. the temperature is 50-60 degree celsius

3.

Polymerisation :Extension of new DNA strands from the primers. The temperature is 72 degree celsius.

Q. Arrange the steps of PCR in decreasing order of temperature.

Denaturation < Polymerisation < Annealing

Polymerisation < Denaturation < Annealing

Polymerisation < Annealing < Denaturation

Annealing < Polymerisation < Denaturation