Alien DNA is inserted in the coding sequence of an enzyme β-galactosidase in artificial cloning vector, a plasmid. How will you select the recombinants in presence of chromogenic substrate?

Question

Alien DNA is inserted in the coding sequence of an enzyme β-galactosidase in artificial cloning vector, a plasmid. How will you select the recombinants in presence of chromogenic substrate? (A) If in the presence of chromogenic substrate the colonies give blue colour then they are recombinants (B) If the colonies do not produce any colour then they are recombinants (C) If the colonies do not produce any colour then they are non-recombinants (D) Both (2) (3)

Tardigrade Admin · Accepted Answer

Correct answer is (b) If the colonies do not produce any colour then they are recombinants

Q. Alien DNA is inserted in the coding sequence of an enzyme $β$ -galactosidase in artificial cloning vector, a plasmid. How will you select the recombinants in presence of chromogenic substrate?

If in the presence of chromogenic substrate the colonies give blue colour then they are recombinants

If the colonies do not produce any colour then they are recombinants

If the colonies do not produce any colour then they are non-recombinants

Both (2) & (3)

Correct answer is (b) If the colonies do not produce any colour then they are recombinants

Q. Alien DNA is inserted in the coding sequence of an enzyme β-galactosidase in artificial cloning vector, a plasmid. How will you select the recombinants in presence of chromogenic substrate?

If in the presence of chromogenic substrate the colonies give blue colour then they are recombinants

If the colonies do not produce any colour then they are recombinants

If the colonies do not produce any colour then they are non-recombinants

Both (2) & (3)

Correct answer is (b) If the colonies do not produce any colour then they are recombinants

Q. Alien DNA is inserted in the coding sequence of an enzyme $β$ -galactosidase in artificial cloning vector, a plasmid. How will you select the recombinants in presence of chromogenic substrate?