A sample of an enzyme called lactase was isolated from the intestinal lining of a calf. Assays were undertaken to evaluate the activity of the enzyme sample. The substrate of lactase is the disaccharide lactose. Lactase breaks a lactose molecule in two, producing a glucose molecule and a galactose molecule. Two assays were carried out. Assay - 1 Lactose concentration (% W/v) 15 15 15 15 15 15 Concentration of enzyme sample (5 v/v) 0 5 10 15 20 25 Reaction rate μ mole glucose sec-1 mL-1 0 25 50 75 100 125 Assay - 2 Lactose concentration (% W/v) 0 5 15 20 25 30 Concentration of enzyme sample (5 v/v) 5 5 5 5 5 5 Reaction rate μ mole glucose sec-1 mL-1 0 15 25 35 40 40 Which of the following assays would you expect to have the highest reaction rate? Lactose concentration (% w /v ) Concentration of enzyme sample (% v/v) (a) 15 5 (b) 30 5 (c) 15 25 (d) 30 25

Question

A sample of an enzyme called lactase was isolated from the intestinal lining of a calf. Assays were undertaken to evaluate the activity of the enzyme sample. The substrate of lactase is the disaccharide lactose. Lactase breaks a lactose molecule in two, producing a glucose molecule and a galactose molecule. Two assays were carried out. Assay - 1 Lactose concentration (% W/v) 15 15 15 15 15 15 Concentration of enzyme sample (5 v/v) 0 5 10 15 20 25 Reaction rate μ mole glucose sec-1 mL-1 0 25 50 75 100 125 Assay - 2 Lactose concentration (% W/v) 0 5 15 20 25 30 Concentration of enzyme sample (5 v/v) 5 5 5 5 5 5 Reaction rate μ mole glucose sec-1 mL-1 0 15 25 35 40 40 Which of the following assays would you expect to have the highest reaction rate? Lactose concentration (% w /v ) Concentration of enzyme sample (% v/v) (a) 15 5 (b) 30 5 (c) 15 25 (d) 30 25  (A) a (B) b (C) c (D) d

Tardigrade Admin · Accepted Answer

Correct answer is (c) c

Lactose concentration (% W/v)	15	15	15	15	15	15
Concentration of enzyme sample (5 v/v)	0	5	10	15	20	25
Reaction rate $μ$ mole glucose $se c^{- 1} m L^{- 1}$	0	25	50	75	100	125