1. Tardigrade
  2. Question
  3. Biology
  4. You take E.coli that has grown in a medium containing only heavy nitrogen ( 15 N ) and tranfer a sample to a medium containing light nitrogen ( 14 N ). After allowing time for two generation, you centrifuge the sample in cesium chloride density gradient. Which band location would support the semiconservative DNA replication after two generations?

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Q. You take E.coli that has grown in a medium containing only heavy nitrogen $\left({ }^{15} N \right)$ and tranfer a sample to a medium containing light nitrogen $\left({ }^{14} N \right)$. After allowing time for two generation, you centrifuge the sample in cesium chloride density gradient. Which band location would support the semiconservative DNA replication after two generations?

Molecular Basis of Inheritance

Solution:

Correct answer is (c) Two DNAs $\left( N ^{14} N ^{15}, N ^{14} N ^{15}\right)$ form