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  4. Which one of the following will be the ratio of heavy, intermediate and light bands in Meselson and Stahl’s experiment after two generations if DNA replication were conservative?

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Q. Which one of the following will be the ratio of heavy, intermediate and light bands in Meselson and Stahl’s experiment after two generations if DNA replication were conservative?

KVPYKVPY 2019

Solution:

The Meselson and Stahl's experiment showed that DNA in replicated by semi-conservative mechanism. In conservative replication, on daughter duplex consist of two newly synthesised strand and parental start is conserved. Parental DNA grown is ${ }^{15} N$ medium (heavy isotope of nitrogen) when centrifuged in first and second generation produces only light and heavy band because parental DNA contains ${ }^{15} N$ in DNA and newly synthesised contain ${ }^{14} N$ in bases. No intermediate density band is formed. Intermediate band is formed in semi-conservative and dispersive replication, where one DNA strand contains ${ }^{15} N$ and other strand contains ${ }^{14} N$.
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