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  4. Which of these is NOT absolutely necessary for a cloning vector?

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Q. Which of these is NOT absolutely necessary for a cloning vector?

NTA AbhyasNTA Abhyas 2020Biotechnology : Principles and Processes

Solution:

The cloning vectors should have a) an Ori which is the origin of replication. b) a Selectable marker c) cloning sites. The commonly used vectors are E coli, plasmids, etc. Artificially prepared vectors are puC and pBr322.If one wants to recover many copies of the target DNA it should be cloned in a vector whose origin support high copy number. But cloning vectors with low copy numbers can also be used. High copy number is not absolutely necessary.