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  4. Which of the following is not a restriction endonuclease?

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Q. Which of the following is not a restriction endonuclease?

Haryana PMTHaryana PMT 2009

Solution:

Restriction endonucleases are the enzymes, which cut a DNA molecule within certain specific sites that have specific base sequence, eg, Hae-III, Eco-RI, Bam-II, Hind-Ill, Pst-I, etc. DNAse-I is not a restriction endonuclease enzyme. DNA polymerase-III synthesizes DNA, while DNA polymerase-I erases primer and fills gaps during DNA replication.