Q. Which of the following enzymes is used to remove the phosphate group from the 5' end of DNA molecule, leaving a free 5' hydroxyl group, so that it cannot be ligated to another DNA fragment?
NTA AbhyasNTA Abhyas 2020
Solution:
Alkaline phosphatase is an enzyme that is used to remove the phosphate group from the 5' end of DNA molecule leaving a free 5' hydroxyl group to prevent any unwanted ligation during the cloning process.
DNA ligase is an enzyme which can join two strands of DNA together by forming a bond between the phosphate and deoxyribose groups on either of the strands.
DNA polymerase is an enzyme involved in the synthesis of DNA molecules from deoxyribonucleotides.
A restriction endonuclease is an enzyme that cleaves the DNA molecule at, or near to, a specific nucleotide sequence to produce discrete DNA fragments.
DNA ligase is an enzyme which can join two strands of DNA together by forming a bond between the phosphate and deoxyribose groups on either of the strands.
DNA polymerase is an enzyme involved in the synthesis of DNA molecules from deoxyribonucleotides.
A restriction endonuclease is an enzyme that cleaves the DNA molecule at, or near to, a specific nucleotide sequence to produce discrete DNA fragments.