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  4. What is the reason of formation of embryoid from pollen grain in tissue culture medium

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Q. What is the reason of formation of embryoid from pollen grain in tissue culture medium

AIPMTAIPMT 2002Strategies for Enhancement in Food Production

Solution:

Totipotency refers to the capacity of each living cell of a multicellular organism being capable of independent development when provided with suitable conditions. Guha and Maheshwari (1966) cultured anthers of Datura. The haploid pollen grain inside the anther divides to form a multicellular mass of cells called callus. Using varying quantities of auxins and cytokinins in the culture medium, this callus is differentiated into embryoids which later germinates to form the entire plant (figure). fig- : Differentiation of embryoids from pollen grains in culture.