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Q.
To prove that $DNA$ is the genetic material, which radioactive isotopes were used by Hershey and Chase $(1952)$ in their experiments?
Molecular Basis of Inheritance
Solution:
Hershey and Chase experiment is based on the fact that $DNA$ contains phosphorus and similarly sulphur is present in proteins but not in $DNA$. They incorporated radioactive isotope of phosphorus ($^{32}P$) into phage $DNA$ and that of sulphur ($^{35}S)$ into proteins of a separate phage culture. Viruses grown in the presence of radioactive phosphorus contained radioactive $DNA$ but not radioactive protein because $DNA$ contains phosphorus but proteins do not. Similarly, viruses grown on radioactive sulphur contain radioactive protein but not radioactive $DNA$ because $DNA$ does not contain sulphur.