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  4. The speed of migration of ions in an electric field during gel electrophoresis depends on

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Q. The speed of migration of ions in an electric field during gel electrophoresis depends on

NTA AbhyasNTA Abhyas 2020Biotechnology : Principles and Processes

Solution:

Electrophoresis is a technique of separation of charged molecules under the influence of an electrical field so that they migrate in the direction of electrode bearing the opposite charge, viz., positively charged molecules move towards cathode (-ve electrode) and negatively charged molecules travel towards anode (+ve electrode) through a medium/matrix. This technique was developed by A. Tiselius in 1937. Any charged ion or molecule migrates when placed in an electric field. The rate of migration depend upon its net charge, size, shape and the applied electric current.