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  4. The presence of a chromogenic substrate gives blue coloured colonies of bacteria. Insertional inactivation would stop production of which of the following enzyme such that colonies do not produce any colour.

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Q. The presence of a chromogenic substrate gives blue coloured colonies of bacteria. Insertional inactivation would stop production of which of the following enzyme such that colonies do not produce any colour.

NTA AbhyasNTA Abhyas 2020Biotechnology : Principles and Processes

Solution:

In PUC plasmid, Lac Z gene is used as a selectable marker. If a transformant is grown in a medium having chromogenic substrate it produces enzyme Beta-galactosidase due to which blue coloured colonies are produced. Insertion of foreign DNA segment in Lac Z gene fails to produces enzyme Beta-galactosidase as a result colourless colonies are produced.