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  4. The limit of resolution of microscope, if the numerical apertura of microscope is 0.12, and the wavelength of light used is 600 nm, is

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Q. The limit of resolution of microscope, if the numerical apertura of microscope is 0.12, and the wavelength of light used is 600 nm, is

Wave Optics

Solution:

For a microscope, the limit of resolution is given by,
$
X =\frac{\lambda}{2 A }
$
where $\lambda$ is the wavelength of light used, and $A$ is the numerical aperture. Hence, substituting the values, $X=\frac{600}{2 \times 0.12}$,
which gives, $X=2.5 \mu m$