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  4. The figure shows the restriction enzyme cutting sites (R1-R3) in wild type (n) and mutant (n) gene. <img class=img-fluid question-image alt=image src=https://cdn.tardigrade.in/img/question/biology/b2a75159f8067305ede4a99438176654-.jpeg /> If a radioactively labelled probe (that hybridises at a sequence close to R1) is used for detecting the presence of DNA fragments after gel electrophoresis and Southern blotting, which of the following band patterns will your expect? Note: L1: wild type DNA, L2: mutant DNA

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Q. The figure shows the restriction enzyme cutting sites $(R1-R3)$ in wild type $(n)$ and mutant $(n)$ gene.
image
If a radioactively labelled probe (that hybridises at a sequence close to $R1$) is used for detecting the presence of $DNA$ fragments after gel electrophoresis and Southern blotting, which of the following band patterns will your expect?
Note : $L1$ : wild type $DNA$, $L2$ : mutant $DNA$

Biotechnology : Principles and Processes

Solution:

Restriction enzymes recognise the base sequence of palindrome sites in $DNA$ duplex and cut its strands. After the cutting of $DNA$ by restriction enzymes, fragments of $DNA$ are formed. Separation of $DNA$ fragments according to their size is done by gel electrophoresis. Since the probe hybridises close to $R1$ it will give $500 \,kb$ band for $L1$ and $400\, kb$ band for $L2$ after gel electrophoresis.