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Q.
The diffraction effects in a microscopic specimen become
important when the separation between two points is
Wave Optics
Solution:
When the wavelength of light used is comparable with the separation between two points, the image of the object will be a $\phi$ diffraction pattern whose size will be
$\theta=\frac{1.22 \lambda}{D}$
where $\lambda=$ wavelength of light used
$D=$ diameter of the objective
Two objects whose images are closer than this distance, will not be resolved.