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  4. The denaturation process in Polymerase Chain Reaction (PCR) does not involve

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Q. The denaturation process in Polymerase Chain Reaction (PCR) does not involve

NTA AbhyasNTA Abhyas 2022

Solution:

PCR stands for Polymerase Chain Reaction. In this reaction, gene (segment of DNA) of interest is synthesised in vitro and multiple copies are generated. The main steps of PCR-
Denaturation - where the double-stranded template DNA is heated to separate it into two single strands.
Annealing - where the temperature is lowered to enable the DNA primers to attach to the template DNA.
Extension - where the temperature is raised and the new strand of DNA is made by the Taq polymerase
enzyme. Thus, option C is correct as it is part of extension step and not denaturation.