Thank you for reporting, we will resolve it shortly
Q.
The acid-soluble pool formed during the chemical analysis of living tissue would have all, except which of the following?
NTA AbhyasNTA Abhyas 2020
Solution:
Different chaemical analyses are performed in order to determine the type of compounds which constitute the living tissue. One such method is chemical analysis using trichloroacetic acid. The sample taken, for example, a piece of liver is properly grinded in acid and filtered with the cotton cloth or cheesecloth. The filtrate contains several chemical compounds of both organic and inorganic, those are acid-soluble. The chemical profiling finds that the acid-soluble fraction contains thousands of mainly small-sized organic compounds.
The characteristics of acid-soluble pool:
- represents roughly the cytoplasmic composition
- contains molecules of smaller sizes, both organic and inorganic (such as phosphates)
- monomeric units of macromolecules are also found, for example; amino acids, glucose, fructose, nucleosides, nucleotides, precursor molecules of lipids etc.
- many types of precursor molecules and their derivatives.
The acid-insoluble fraction has molecules of larger sizes such as - nucleic acids: DNA and RNA
- proteins and
- polysaccharides: glycogen, starch.
These macromolecules (size equal or larger than 1,000 Da except) do not mixed in the acid solution and remained in the retentate.
Besides these molecules, acid-insoluble pool also contains cell organelles or broken pieces of cell membrane and organelles, also the vesicles are formed. The lipids are found in the form of vesicles obtained from the retentate.